Ultrasonic Homogenizer Published Papers
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| Article No. | Title |
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| UH1091 | Increased yield of high purity biologically active recombinant human granulocyte-macrophage colony-stimulating factor from Escherichia coli |
| Chitra Bajji1,2*, Ravi Kanth Reddy Kosana1, Radha Madhavi Kanumuri1 and Murali Krishna Reddy Tummuru1 | |
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Human Granulocyte-Macrophage Colony-Stimulating Factor (hGM-CSF) is being produced by recombinant DNA technology and has been used as a therapeutic protein for different conditions. Different strategies have been reported to express and purify therapeutic grade hGM-CSF in different host systems including Escherichia coli. However, the reported yields are comparatively low and involve multiple steps of purification. In this present paper, we report a novel method of periplasmic secretion system for high expression, with relatively simple purification method using reversed phase chromatography and refolding technique. The final purity of the rhGM-CSF was more than 98 % with increased yield of ~300 mg per 35 g of wet weight cell pellet per litre of culture, which is relatively higher than the previous reports. Moreover, the final purified rhGM-CS proteinF has exhibited specific biological activity (1.1×107 IU/mg) in TF-1 cell proliferation assay similar to standard protein. |
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| UH1092 | Effects of Media Composition on the Production of Linamarase from Lactobacillus delbrueckii NRRL B-763 |
| Ogbonnaya Nwokoro and Florence Onyebuchi Anya | |
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Nutritional factors relating to the production of linamarase from Lactobacillus delbrueckii NRRL B-763 were investigated. The microorganism was cultivated in a medium containing 1% linamarin. Enzyme was produced using a variety of carbon substrates but the highest enzyme activity was detected in the presence of salicin ( 522 U/ml) after 48 h while the lowest yield was observed with carboxyl methyl cellulose (38 U/ml) after 72 h. Enzyme was not produced in the presence of cellobiose. Among a variety of nitrogen substrates tested, peptone supported maximum enzyme production (412 U/ml) after 48 h. Lowest enzyme production was observed with urea (40 U/ml). Organic nitrogen substrates generally supported higher enzyme productivity than inorganic nitrogen substrates. Enzyme activity was observed in the presence of Mn2+ (% relative activity = 216) while Hg2+ was inhibitory (% relative activity = 28). Locally - formulated media were comparable to MRS broth in supporting linamarase production by the bacterium. Higher enzyme activity was produced in media with surfactant than in media without surfactant. The enzyme may be useful in enhanced degradation of cassava cyanide. |
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| UH1093 | Dopamine neuron dependent behaviors mediated by glutamate cotransmission |
| Susana Mingote, Nao Chuhma, Abigail Kalmbach, Gretchen M Thomsen, Yvonne Wang, Andra Mihali, Caroline Sferrazza, Ilana Zucker-Scharff, Anna-Claire Siena, Martha G Welch, Jose´ Lizardi-Ortiz, David Sulzer, Holly Moore, Inna Gaisler-Salomon, Stephen Rayport | |
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Dopamine neurons in the ventral tegmental area use glutamate as a cotransmitter. To elucidate the behavioral role of the cotransmission, we targeted the glutamate-recycling enzyme glutaminase (gene Gls1). In mice with a dopamine transporter (Slc6a3)-driven conditional heterozygous (cHET) reduction of Gls1 in their dopamine neurons, dopamine neuron survival and transmission were unaffected, while glutamate cotransmission at phasic firing frequencies was reduced, enabling a selective focus on the cotransmission. The mice showed normal emotional and motor behaviors, and an unaffected response to acute amphetamine. Strikingly, amphetamine sensitization was reduced and latent inhibition potentiated. These behavioral effects, also seen in global GLS1 HETs with a schizophrenia resilience phenotype, were not seen in mice with an Emx1-driven forebrain reduction affecting most brain glutamatergic neurons. Thus, a reduction in dopamine neuron glutamate cotransmission appears to mediate significant components of the GLS1 HET schizophrenia resilience phenotype, and glutamate cotransmission appears to be important in attribution of motivational salience. |
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| UH1094 | Preparation, Characterisation and In vivo Evaluation of Bis-demethoxy Curcumin Analogue (BDMCA) Nanoparticles |
| CA Anuradha and Jithan Aukunuru | |
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To fabricate biodegradable nanoparticle formulation of bis-demethoxy curcumin analogue (BDMCA), a novel curcumin analogue, and evaluate its in vitro and in vivo characteristics. Methods: Nanoparticle formulations were fabricated by a double emulsion solvent evaporation technique using polycaprolactone as the polymer. The nanoparticles were characterised for drug content, particles size, in vitro drug release and the drug-polymer interaction. The in vivo properties of the formulations in male Wistar rats were evaluated from the pharmacokinetics and pharmacodynamics of BDMCA following i.v. administration of the nanoparticles. BDMCA solution was administered i.v. as a reference. Hepatoprotectivity of the formulation was determined in a CCl4-treated rat model. |
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| UH1095 | Characterization of prodigiosin produced by Serratia marcescens strain isolated from irrigation water in Egypt |
| Ahmed H. Faraag; Ahmed I. El-Batal and Hoda H. El-Hendawy | |
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The spectroscopic analyses of the red pigment with UV–vis spectral analysis, Gas chromatography–mass spectrometry (GC/MS), Fourier-Transform Infrared Spectroscopy (FTIR Spectroscopy) and IH-Nuclear Magnetic Resonance Spectroscopy (NMR Spectroscopy), indicated that the red pigment produced by Serratia marcescens strain WSE is prodigiosin. The cluster genes (pig cluster) responsible for production of prodigiosin by S. marcescens strain WSE were amplified by PCR, sequenced and their nucleotide sequences were analyzed through bioinformatics. High degree of similarity was detected between the nucleotide sequence of the pig clusters and the nucleotide sequence of S. marcescens in the NCBI database (Acc. No. AJ833002, Acc. No. CP005927 and Acc. No. CP003959). Effect of precursor amino acids on prodigiosin production by S. marcescens isolate WSE were examined and the results revealed that maximum pigment production was obtained by growing S. marcescens in Nutrient Broth supplemented with 10 mg/ml L-tyrosine. This study suggests that L-Tyrosine plays an important role for maximum induction of pigment production by S. marcescens strain WSE. We describe a new method for estimation of prodigiosin concentrations intra and extracellular. We also report the organization of prodigiosin biosynthetic gene (pig) clusters in S. marcescens strain WSE based on predicted protein functions of the genes of the pig clusters. |
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| UH1096 | IL-1ra delivered from poly(lactic-co-glycolic acid) microspheres attenuates IL-1b-mediated degradation of nucleus pulposus in vitro |
| Deborah J Gorth, Robert L Mauck, Joseph A Chiaro, Bhavana Mohanraj, Nader M Hebela, George R Dodge, Dawn M Elliott and Lachlan J Smith | |
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Inflammation plays a key role in the progression of intervertebral disc degeneration, a condition strongly implicated as a cause of lower back pain. The objective of this study was to investigate the therapeutic potential of poly(lactic-co-glycolic acid) (PLGA) microspheres loaded with interleukin-1 receptor antagonist (IL-1ra) for sustained attenuation of interleukin-1 beta (IL-1b) mediated degradative changes in the nucleus pulposus (NP), using an in vitro model. |
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| UH1097 | Early-life perturbations in glucocorticoid activity impacts on the structure, function and molecular composition of the adult zebrafish (Danio rerio) heart |
| K.S. Wilson, J. Baily, C.S. Tucker, G. Matrone, S. Vass, C. Moran, K.E. Chapman, J.J. Mullins, C. Kenyon, P.W.F. Hadoke, M.A. Denvir | |
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Transient early-life perturbations in glucocorticoids (GC) are linked with cardiovascular disease risk in later life. Here the impact of early life manipulations of GC on adult heart structure, function and gene expression were assessed. |
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Ultrasonic Homogenizers
Sometimes referred to as, Sonicator, Cell Disruptor or Cell Disrupter, Probe Sonicator, Ulrasonicator, Sonifier®, Sonic Dismembrator, and Ultrasonic Liquid Processor.
Sonifier is a registered trademark of Branson Ultrasonics Corporation
