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ULTRASONIC HOMOGENIZER APPLICATION NOTES

| Model 150VT | Model 300VT | Model 3000 | Model 3000MP |
| Tips and Accessories | Application Notes | Published Papers |

| A | B | C | D | E | F | G | H |
| I | J | K | L | M | N | O | P | Q | R | S |
| T | U | V | W | X,Y,Z |

Alphabetical Listing ( T - Z )

-T-

Tablets - complete disruption in 2-40 seconds depending on type. Excellent for automatic machines.

Tea - excellent extraction.

Tetrahymena - disrupts in a few seconds. Enzymes which have been monitored include: succinate, lactate, B-hydroxy butyrate, glutamate and DPNH oxidase, DPNH-cytochromeC reductase and ribonuclease. Specify activity of DPNH oxidase was twice that of the best previous experiments.

Thermoactinomyces - disruption of hyphae. Homogenization of protein complex without denaturation.

Thermophile negative - good disruption with in 2 minutes.

Thermophilic bacillus - Isocitrate lyase was extracted from a spore forming bacillus similiar to Stearothermophilus. A washed cell paste suspended in phosphate buffer was sonicated in 1-2 minutes and the supernatant was used for enzyme experiments without further treatment. Extracts could be frozen and stored without loss of activity.

Thiouric acid - dissolved in a few seconds.

Thymus cells - complete disruption in 15 seconds

Tissue cultue cells - complete disruption in a few seconds. To avoid damage to free organelles and to obtain intact lyososomes, use low power at short exposure.

Toxin and antitoxin - one example of many: Toxin preparations of whole cell lystae (WCL) of the Inaba serotype strain 569E of the classic biotype of cholera vibrio were grown on 3% Bacto peptane agar and harvested in distilled water at 18 hours.

Toxoplasma gondi i - can be seoarated from white blood cells without injuring.

T. pyriformis - excellent disruption; 8 enzymes released.

Transplantation antigens - were extraced from spleen, thymus and lymph nodes.

Trichomonas foetus - complete disruption in a few seconds.

Triolein - complete stable emulsion in 2 minutes.

Trypanosomes - concentrated 10 ml solution; complete disruption in 4 minutes.

Tumor tissue - disintegrates much faster than normal tissue.

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-U-

Uterus muscle - 1/5 gm, 3 cc solution; complte disruption in 4 minutes for coenzyme Q determination.

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-V-

Vaccines - numeous advantages such as more antigenic material released than usual and the producing of vaccines not obtainable by classical methods.

Variuos bacilli - complete disruption in 3 minutes.

Vibrio comma - excellent disruption.

Virus extraction - excellent for making experimental vaccines. Evidence of breakage of virus/antibody bonds. Virus ban be extracted at low power without damage, or broken at high power.

Vitamin E - 30 second sonication put material in solution with a resultant permanent suspension.

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-W-

W138 Virus - Cell free V-2 virus obtained in 30 seconds using 6 ml of Veronal buffer with W138 cells containing V-2 virus.

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-X,Y,Z-

Yeast - 3 to 10 minutes for complete disruption depending on type.

Zooplankton - disrupted in less than 1 minute.

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Ultrasonic Homogenizers
Sometimes referred to as, Sonicator, Cell Disruptor or Cell Disrupter, Probe Sonicator, Ulrasonicator, Sonifier®, Sonic Dismembrator, and Ultrasonic Liquid Processor.

Sonifier is a registered trademark of Branson Ultrasonics Corporation

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BIOLOGICS MANUFACTURES ULTRASONIC HOMOGENIZERS

Our the Ultrasonic Homogenizer product line is being expanded to include the MultiSonic and AutoSonic, both robotic systems, for multi sample processing of volumes ranging from 250 µl to 50 ml. 

Titanium Tips and Accessories were also expanded to allow processing of a wider range of sample volumes.

Ultrasonic Homogenizers are also referred to as Sonicators, Cell Disruptors, Probe Sonicators, Sonifiers®, and Sonic Dismembrators

Sonifier is a registered trademark of Branson Ultrasonics Corporation

 
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 BioLogics, Inc.
Manassas, Virginia 20109
United States of America

Tel: (703) 367-9020
Fax: (703) 367-9024
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